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Thursday, May 19, 2011

RHH mapper: results for V158-V165 and V201-V202

RHHmapper allows users to visualize chromosomal mosaicism in admixed individuals descended from two genetically distinct groups.
Chromosomal mosaicism is when different cells within an individual, who has developed from a single fertilized egg, have a different chromosomal makeup. Most commonly there will be some cells with a typical number of chromosomes (46 chromosomes) and other cells with an altered number or structure of chromosomes.

RHH Counter (Rare Heterozygotes and Homozygotes) is a command line application designed to display the chromosomal mosaicism in admixed individuals as parf of two genetically distinct groups. The software also detects "outliers" whose ancestry is different or admixed compared to most other subjects in a dataset.

I wouldn't mind writting the extensive description of this tool, but due to some sort of time deficiency, i would rather pull some words from editor's description. Also, RHHcounter has been already discussed by Davidski in his Eurogenes blog(explaining how it's possible to get the most out of the RHHcounter/RHHmapper data using SPSmart)

Despite its evident limitations (which have been made explicit by certain persons), the method could be useful for detecting both remote signals of ancient admixture events and more recent "geneographic" traces. For the latter type of analysis, we need some heuristic method to extrapolate the size of shared segments in 23andme's Ancestry Finder.

In order to illustarte the concept, I will use Ancestry Finder's data of my own family members:

VV


LKH (VV's mother)


Next, we need to compare RHH mappings, looking for rare hetero- or homozygotes shared between those two family members.






The common RH for VV and LKH is indicated by a blue mark in Chr.4 track. A blue mark indicates that a rare homozygous genotype was observed in this sample across at this position. In most cases, rare homozygotes are less common than rare heterozygotes. However, the presence of rare homozygotes can be informative of the level of admixture in a particular chromosomal region. VV (LKH's son) also have rare heterozygotes on Chr.7 and Chr.13.

The next logical step is to compare detected rare RHH to Ancestry Finder's data. Since the exact bounds of the shared IBD segments (as shown in AF) are uncertain, we must search for the segment (and geneographic data, associated with those segments) in physical locations closest to RHH's location. For instance, in our example one may be tempited to show that RHH can be attributed to the extended genetic pool of Polish, Hungarian, Lithuanian and Czech populations.




The complete set of chromosomal mosaicisms for all project's participants is available for download here.

P.S. More interesting is, however, the combination of rare heterozygotes on Chr.7 (between 110Mb and 120Mb +-10mb) and Chr.13 (between 40Mb and 50Mb +-10Mb) tends to be prevalent (excluding two Romanians) in Uzbeks and Turks. Even more interesting fact is that one of VV' great-great-great-grandfathers came of a noble Tatar family which had been settled in Grand Duchy of Lithuanie before 1528.

2 comments:

  1. Very cool. If there's time and computing power I would like to see analysis on all the chromosomes including the X.

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  2. Yes, i probably will.
    But i would like to remind that this test was done using a previous sample numbering system.
    You, and all new participants from the past week are not included in this analysis (the number refers to another sample).
    Since then i have changed the numbering of samples in such a way that is consistent with ID number you got on the last week.

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